5-HIAA

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Also known as: 5-hydroxyindoleacetic acid; HIAA; Serotonin metabolite
Formal name: 5-hydroxyindoleacetic acid
Related tests: Serotonin, Chromogranin A

At a Glance

Why Get Tested?

To help diagnose and monitor treatment for a serotonin-secreting carcinoid tumour

When to Get Tested?

When you have symptoms suggestive of a carcinoid tumour such as flushing, diarrhoea, and/or wheezing; at intervals following treatment

Sample Required?

Test Preparation Needed?

You may be instructed to avoid certain foods and medications prior to this test. Please follow any instructions you are given.

The Test Sample

What is being tested?

This test measures the amount of 5-hydroxyindoleacetic acid (5-HIAA) in the urine. 5-HIAA is a muscle stimulant and the primary metabolite of serotonin, a chemical derived from the amino acid tryptophan. In health, most serotonin is produced by the gastrointestinal (GI) tract where it regulates intestinal movements. The remainder is produced by the central nervous system where it functions to regulate mood, appetite and the wake/sleep cycle. After it has been used by the body, serotonin is broken down in the liver, and its metabolites, including 5-HIAA, are excreted in the urine. Normally, only small amounts of 5-HIAA is present in the urine. However, large quantities of serotonin and 5-HIAA may be produced by some carcinoid tumours.

Carcinoid tumours are typically slow-growing masses. The majority of carcinoid tumours form in the gastrointestinal tract but they can also form at other sites such as in the lungs and the pancreas. Many carcinoid tumours remain small and do not cause any symptoms. A small percentage of carcinoid tumours grow large enough to cause obstructions in the intestines or bronchial tubes of the lungs. Some carcinoid tumours can spread to other organs. The most common site of spread is to the liver.

About 10% of carcinoid tumours, primarily those found in the gastrointestinal tract, will produce enough serotonin to cause symptoms such as flushing of the face, diarrhoea, a rapid heart rate, and wheezing. This collection of symptoms is referred to as the carcinoid syndrome. Such symptoms will usually only appear after the tumour has spread to the liver. The serotonin that causes the carcinoid syndrome may be released continuously or intermittently and can lead to significantly increased quantities of 5-HIAA in the urine.

How is the sample collected for testing?

For the 24-hour urine collection, all urine should be saved for a 24-hour period. It is better to keep sample in a cool dark place. When complete the sample is taken as soon as possible to your GP or the laboratory for preservation. Pre-sample preparation is important for accurate 5-HIAA test results.
For more information, see Is there anything else I should know and talk to your doctor.

NOTE: If undergoing medical tests makes you or someone you care for anxious, embarrassed, or even difficult to manage, you might consider reading one or more of the following articles: Coping with Test Pain, Discomfort, and Anxiety, Tips on Blood Testing, Tips to Help Children through Their Medical Tests, and Tips to Help the Elderly through Their Medical Tests.

Another article, Follow That Sample, provides a glimpse at the collection and processing of a blood sample and throat culture.

Is any test preparation needed to ensure the quality of the sample?

Pre-sample preparation is important for accurate 5-HIAA test results. Foods such as avocados, bananas, pineapples, plums, walnuts, tomatoes, kiwi fruit, and aubergine (eggplant) can interfere with 5-HIAA measurement and should be avoided for 3 days prior to and during urine collection. There are also a variety of drugs that can affect the 5-HIAA test. It is important that those being tested talk to their healthcare provider before decreasing or discontinuing any medications.

The Test

Common Questions

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NOTE: This article is based on research that utilizes the sources cited here as well as the collective experience of the Lab Tests Online Editorial Review Board. This article is periodically reviewed by the Editorial Board and may be updated as a result of the review. Any new sources cited will be added to the list and distinguished from the original sources used.